The aim of the current study was to verify whether the specific kickboxing circuit training protocol (SKCTP) could reproduce kickboxing combat's hormonal, physiological and physical responses. Twenty athletes of regional and national level volunteered to participate in the study (mean±SD) age: 21.3±2.7 years, height: 170±0.5 cm, body mass: 73.9±13.9 kg. After familiarisation, SKCTP was conducted one week prior to a kickboxing competition. Cortisol, testosterone, growth hormone (GH), blood lactate [La] and glucose concentrations, as well as Wingate upper body test, countermovement jump (CMJ) performances were measured pre and post SKCTP and combat. Heart rate (HR) and rating of perceived exertion (RPE) were measured throughout rounds (R) R1, R2 and R3. Testosterone, GH, glucose, [La], HR, RPE and CMJ did not differ among the two conditions (P>0.05). However, Cortisol was higher for competition (P =0.038), while both peak (P =0.003) and mean power (P <0.001) were higher in SKCTP. The study suggests that SKCTP replicates the hormonal, physiological and physical aspects of competition. It is therefore suggested as a good form of specific kickboxing training, as well as a specific assessment tool to be used by kickboxing coaches to quantify kickboxers' fitness levels, when physiological parameters responses to the test are measured.